学生姓名：

教师顾问(s):

林康·迪亚兹，丹尼尔

Ansul Lokdarshi博士

摘要标题

Methyl jasmonate stress signaling in 拟南芥 functions via reactive oxygen species to activate the GCN2-eIF2α module

文摘:

植物的生长和生产力依赖于快速的能量管理策略，这些策略旨在应对动态环境条件（例如，波动的光强、温度、湿度和病原体的相互作用）。 Previous work by Lokdarshi et al., identified a novel fast-regulatory switch in 拟南芥 that functions at the nexus of two fundamental energy management programs, cytosolic translation and reactive oxygen species (ROS) signaling. 研究表明，胞质丝氨酸/苏氨酸蛋白激酶GCN2 （General Control of nonderexsible 2）在各种非生物、生物和异种生物胁迫下对ROS的响应迅速激活。 GCN2 then phosphorylates its target, α-subunit of the eukaryotic translation initiation factor (eIF)2, resulting in readjustments to the active protein synthesis as a plausible mode for stress remediation. In the work presented here, we test the hypothesis that the biochemical, molecular and physiological responses of the Arabidopsis GCN2-eIF2α module towards the plant defense hormone, methyl jasmonate (MeJA) is regulated by light and ROS. We show that eIF2α phosphorylation (P-eIF2α) as proxy for GCN2 activation under MeJA stress requires light and this activation can be mitigated with antioxidants and photosynthetic inhibitors. At the physiological level, gcn2 mutant seedlings show increased sensitivity towards MeJA stress in a primary root growth assay. Interestingly, gcn2 mutant shows similar rate of protein synthesis as the wild-type under MeJA stress as evidenced by polysome profiling and puromycin incorporation assay. Taken together, we show the conservation of Arabidopsis GCN2-eIF2α activation by ROS during methyl jasmonate stress.

学生姓名： 

教师顾问(s):

贝拉，这是艾德里安·L。

Theodore A. Uyeno博士

学生姓名：

教师顾问(s):

肯雅娜·泰勒。

Emily Cantonwine博士

摘要标题

硫合成杀菌剂对花生叶斑病和锈病的防治效果比较

文摘:

Early and late leaf spot, caused by the fungi Passalora arachidicola and Nothopassalora personata, are important foliar diseases of peanut that can lead to premature defoliation and yield loss if not controlled. In fields with a history of leaf spot, the diseases are managed using frequent applications of fungicides. Recently, mixtures of micronized elemental sulfur with tebuconazole, a demethylation inhibitor (DMI) fungicide, or azoxystrobin, a quinone outside inhibitor (QOI) fungicide,were shown to lessen defoliation caused by leaf spot. The objective of this study was to determine if sulfur mixtures reduce defoliation by causing fewer leaf spot infections or byincreasing plant tolerance. Field studies conducted at the University of Georgia Coastal Plain Experiment Station in 2020 and 2021 exposed peanut plants to 7 applications of six fungicide treatments at recommended rates: (1) a nontreated control, (2) sulfur, (3) tebuconazole (DMI), (4) DMI + sulfur, (5) axozystrobin (QoI), and (6) QoI + sulfur. Disease was assessed weekly between 90 and 140 days after plating (dap) using the FL 1-10 scale, a visual estimation of defoliation and disease severity, and as the number of leaflets with leaf spot (leafletincidence) and the mean number of lesions per leaflet (leaflet severity) for 10 lateral branches per plot. As expected, defoliation assessments were significantly lower for the sulfur mixture treatments than the DMI and QoI treatments alone in most cases (P<0.05). 小叶发生率和严重程度也有相似的规律（P<0.05）。 There was a significant exponential relationship between cumulative leaflet severity(AUDPC severity) and final defoliation (R² = 0.77; P<0.001) and AUDPC severity and AUDPC defoliation (R² = 0.82; P<0.001). 在大多数情况下，当AUDPC严重程度作为协变量纳入模型时，硫对落叶的影响不显著（P < 0.05）。 This suggests that the mechanism of reduced defoliation is due to fewer leaf spot infections rather than increased plant tolerance.

Additional studies were conducted to evaluate the effects of sulfur mixtures on rust disease, caused by 柄锈菌arachidis, under field conditions, and on the growth of N. personta in culture. 与单独使用DMI和qi相比，混合硫对铁锈严重程度没有显著改善（P < 0.05）。 Results from the 在体外 study suggests that sulfur mixtures did not significantly reduce germination compared to the DMI and QoI treatments alone (P=0.14) but may reduce hyphal branching(P<0.05). However, treatment effects on germtube number and total hyphal growth were inconsistent across the two experiments. More research is needed to determine if the mechanism for reduced infections is the result of increased toxicity to the fungus.

学生姓名：指导教师：

勒,克里斯托弗

j.m.洛克哈特博士

摘要标题

Survival assessment and movement analysis of gopher tortoises (Gopherus波吕斐摩斯) in south Georgia, USA

文摘:

地鼠龟（Gopherus波吕斐摩斯）的重要和受威胁的地位使它们成为野生动物管理的一个感兴趣的物种。 在南乔治亚州对地鼠龟进行了两项研究。 第一项研究评估了2008年和2009年在美国乔治亚州库克县里德宾厄姆州立公园（RBSP）放生的174只被动综合应答器（PIT）标记的地鼠龟幼龟的生存能力。 假设有10%（17/174）会被重新捕获。 从2020年3月至2021年7月，对放生地点地鼠龟管理区和先锋地点进行了调查。 174只带pit标记的幼龟中0只（N = 0）被重新捕获。 被捕食、人类存在、检测困难、远离放生地点和上呼吸道疾病是导致再捕获失败的原因。 第二项研究利用全球定位系统（GPS）技术分析了一只成年雄性地鼠龟在穆迪空军基地（位于佐治亚州朗兹和拉尼尔县的军事设施）的运动活动。 从2020年9月到2021年1月，gps追踪了其运动轨迹。 GPS数据包含位置、温度和卫星特定的获取信息。 共获得263个固定位置（N = 263）。 FL were analyzed for home range, core area, distance >300 m, and nocturnal movement (20:00 – 05:00). Home range increased 10-fold with increasing horizontal dilution of precision (HDOP) (range: 3.55 – 15.86 ha, HDOP ≤ 1.5 – 9.9), core area was 0.13 ha, greatest straight-line distance was 345 m, and 41 nocturnal events occurred. GPS unit troubleshooting and gopher tortoises’ fossorial nature attributed to early battery life depletion and FL accuracy and precision analysis. 主要发生在核心区； 核心区域外的长距离运动出现了5次，其中一次包括夜间运动。 远距离迁移的影响因素包括生境质量、越冬挖洞、繁殖、社会交往和能量消耗恢复。

学生姓名：

赫克托耳,Jaelle

詹姆斯·尼诺博士

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学生姓名：

教师顾问(s):

mbeahuruike奇迹O。

克里斯蒂娜·卡莱斯塔尼博士

学生姓名：

达米恩·卡斯特利亚诺

摘要标题

Comparing Nothopassalora personata pre-infection development, colonization, and haustorial characteristics between susceptible and resistant peanut genotypes. 

文摘:

学生姓名：

教师顾问(s):

霍利，安布尔·N。

埃里克·钱伯斯博士

马克·布莱克莫尔博士

j.m.洛克哈特博士

Erin Grabarczyk博士

摘要标题

Disease Surveillance of Dirofilaria巨细胞 and West Nile Virus in Mosquitoes in Lowndes County, Georgia

文摘:

Dirofilaria巨细胞犬心丝虫病的病原体西尼罗河病毒（WNV）都是蚊子传播的病原体。 蚊虫监测和媒介确定对于了解这些病原体的传播至关重要，这有助于实施控制方法，以预防和预测疫情。 In this study I assessed the ability of the 按蚊quadrimaculatus complex to transmit d .巨细胞 and investigated the influence climate and land use and land changes (LULC) have on WNV transmission in 这种致倦库蚊 mosquitoes, the primary vector in the Southeastern United States. Standard PCR and an L3-specific RT-PCR protocol were used to assess d .巨细胞 infection rates in 一个。 quadrimaculatus mosquito samples. 为了确定2012-2021年气候和LULC对西尼罗河病毒的影响，我们收集了来自Weather Underground和vs上西尼罗河病毒蚊子监测实验室的数据。 Results from this study suggest that the 一个。 quadrimaculatus complex is a potential vector of d .巨细胞 and that urbanization and temperature influence WNV transmission by mosquitoes in Lowndes Co., GA.